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After completion of the reaction, the pH was adjusted to pH = 2-3 with citric acid solution (pH = 2)

It is not intended for human consumption , veterinary use, diagnostic use, therapeutic use, clinical use, injury treatment, recovery treatment, or use as a food, drug, cosmetic, supplement, or household product

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Don't compromise on the most fundamental variable in your entire experiment

Comparison of immortalized bEnd5 and primary mouse brain microvascular endothelial cells as in vitro blood-brain barrier models for the study of T cell extravasation